Robust photoelectrochemical cytosensor in biological media using antifouling property of zwitterionic peptide

Key Words:Photoelectrochemical cells;Indium compounds - Lanthanum compounds - Peptides - Tin oxides - TiO2 nanoparticles - Titanium dioxide - Zinc compounds

Abstract:Resulting from nonspecific adsorption or obvious biofouling, target detection in real biological media is a potential challenge for most of the biosensors. Herein, we first report an antifouling photoelectrochemical (PEC) cytosensor by using zwitterionic peptide. The typical cervical carcinoma Henrietta Lacks (HeLa) cell was chosen as a target model and the aptamer AS1411 was used as its recognition element. TiO<inf>2</inf> nanoparticles (NPs) and ZnIn<inf>2</inf>S<inf>4</inf> nanocrystals (NCs) were modified in order on a bare indium–tin oxide (ITO), forming ITO/TiO<inf>2</inf>/ZnIn<inf>2</inf>S<inf>4</inf> electrode as the PEC matrix to immobilize aptamer AS1411 and zwitterionic peptide. The fabricated PEC cytosensor exhibited a high sensitivity toward HeLa cell detection, with the detection limit of 34 cells/mL. Besides, owning to excellent antifouling property of the zwitterionic peptide, evidently reduced nonspecific adsorption of the cytosensor was realized in the biological media.<br/> © 2019 Elsevier B.V.

Volume:299

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Translation or Not:no