关键字：MICRORNA; NANOSTRUCTURE; CANCER
摘要：The early-stage detection of microRNA (miRNA) holds significant value for clinical diagnosis of tumor. In this study, a highly sensitive miRNA detection platform is developed using a photoelectrochemical sensing system based on layer-by-layer (LBL) assembled ZnS/MXene/CdS (ZMC) heterostructure electrode. By incorporating ultrathin and highly conductive MXene, the ZMC electrode exhibits robust photocurrent signals upon exposure to light. The electrode is further modified HS-H1 DNA via disulfide bonds.With the help of auxiliary H2 DNA, a target miRNA-21 triggered catalytic hairpin assembly (CHA) amplification reaction is realized on the electrode surface through complementary base pairing. Then, manganese porphyrin (MnPP) is introduced to the formed double-stranded DNA after CHA reaction to catalyze the conversion of 4-chloro-1-naphthol (4-CN) to benzo-4chloro-hexanedione (4-CD) precipitate in the presence of hydrogen peroxide, which leads to a significant decrease in photocurrent signals. As a result, the sensor demonstrates a reliable linear correlation in miRNA-21 detection with detection limits as low as 0.36 fM. The recoveries of miRNA-21 in human serum samples ranged from 98.27 % to 101.35 % with RSD values below 3.67 % suggest the reliability and practicality of this method. Therefore it shows great potential for the construction and application of highly sensitive photoelectrochemical biosensors in clinical sample detection.
卷号：419
期号：-
是否译文：否