Selective and sensitive biosensors based on metal-enhanced fluorescence
关键字：Metal-enhanced fluorescence; Ag@SiO2; DNA; Fluorescent probe; Target sensing
摘要：A biosensing platform using Ag@SiO2-DNA-fluorophore nanostructure was designed for metal ions and small organic molecules detection. DNA hybridization occurred in the presence of the targets through the formation of base-target-base complex; accordingly, the fluorophore (Cy3) was brought to the surface of Ag@SiO2 core/shell nanostructures. Through controlling the thickness of the silica shell on the Ag nanoparticle, the fluorescence of Cy3 could be enhanced by the surface plasmon resonance of Ag nanoparticle for up to 2.5 folds. This metal-enhanced fluorescence (MEF) sensor provided high sensitivity and selectivity for the detection of Hg2+, Ag+, and coralyne. It may be developed as a new class of MEF biosensors for other metal ions and organic molecules analysis. (C) 2014 Elsevier B.V. All rights reserved.
卷号：202
期号：
是否译文：否
