An Amperometric Biosensor of Determination H2O2 Based on Horseradish Peroxidase in Carbon Nanotubes/Ionic Liquid

• Key Words:Biosensor; Horseradish peroxidase; Muti-walled carbon nanotubes; Ionic liquid; Hydrogen peroxide
• Abstract:A novel amperometric biosensor for the determination of H2O2 based on horseradish peroxidase (HRP) in nanocomposite material of muti-walled carbon nanotubes/ionic liquid was explored. Cyclic voltammetry (CV) was used to characterize the performance of the biosensor. Under the optimized experimental conditions, H2O2 could be detected in a linear calibration range of 0.5 x 10(-6) M similar to 6.0 x 10(-6) M with a correlation coefficient of 0.9902 (n = 7), a detection limit of 1.5 x 10(-7) M at 3 sigma and the recovery ratio was of 96.2% similar to 110.8%, which indicated that the accuracy of this method is also satisfied. The modified electrodes display more excellent electrochemical performance, high sensitivity, good reproducibility, and long-term stability.
• Volume:34
• Issue:6
• Translation or Not:no