关键字：NITRIC-OXIDE; ENDOGENOUS PEROXYNITRITE; HYDROXYL RADICALS; IN-VITRO; VISUALIZATION; DOTS
摘要：As a potent reactive oxygen species (ROS), peroxynitrite (ONOO-) is produced when superoxide anions (O2 center dot- ) react with nitric oxide (NO). This molecule has been demonstrated to be closely linked to the progre of numerous diseases. Peroxynitrite is highly reactive and quite unstable with a very short half-life, which is primarily found within mitochondria. Therefore, creating a mitochondria-targeted fluorescent probe that can selectively detect peroxynitrite is of great importance. In this work, a ratiometric fluorescent probe RHON is designed to detect ONOO-both sensitively and selectively. The probe, featuring an oxabenzofluorene-based structure, was efficiently synthesized in a few short steps. After the addition of ONOO-, the pinkish-purple colour of the probe solution gradually disappeared and finally became colourless. Simultaneously, a substantial blue shift was observed in the fluorescence emi, covering a range of 115 nm. After thorough evaluations, the probe was found to have outstanding selectivity and sensitivity toward ONOO-.Impressively, it can reach an extremely low detection limit of 0.11 nM, and its response time is quite rapid, approximately 1 min. Furthermore, RHON exhibits outstanding biocompatibility. Moreover, the exogenous ONOO-present in mitochondria of living cells could be successfully detected by this probe. This successful application vividly demonstrates the great potential of RHON in the field of biological applications.
卷号：283
期号：
是否译文：否