Electrochemical aptasensor based on Au@HS-rGO and thymine-Hg²⁺-thymine structure for sensitive detection of mercury ion

发布时间：2023-10-19 点击次数：

• 关键字：Hydraulic structures;DNA - Glass membrane electrodes - Mercury (metal) - Voltammetry
• 摘要：A sensitive electrochemical aptasensor was prepared on the basis of gold modified thiol reduced graphene (Au@HS-rGO) and thymine-Hg²⁺-thymine (T-Hg²⁺-T) constitution for the checkout of Hg²⁺. Firstly, Au@HS-rGO was fabricated as the substrate onto the glassy carbon electrode (GCE) surface, which facilitated the subsequent introduction of single-stranded DNA onto the electrode. Following that, thionine (Th) was immobilized on the electrode surface depend on the physical size effect of T and Hg²⁺. Finally, the amount of Hg²⁺ was determined by differential pulse voltammetry (DPV) of Th. Under the best conditions, the linear range of the aptasensor to Hg²⁺ was in the range of 1–200 nmol/L and the detection limit was as low as 0.38 nmol/L. Simultaneously, good selectivity and acceptable reproducibility were revealed in the designed aptasensor. Moreover, the design thought may provide an idea for the detection of other substances in the water sample.<br/> © 2019 Elsevier B.V.
• 卷号：848
• 期号：*
• 是否译文：否