Peptide-based biosensor for the prostate-specific antigen using magnetic particle-bound invertase and a personal glucose meter for readout

发布时间：2021-03-15 点击次数：

• 关键字：Prostate-specific antigen; Personal glucose meter; Peptide; Biosensor; Gold nanoparticles
• 摘要：We describe a highly sensitive and selective peptide-based biosensor for the prostate-specific antigen (PSA). The biotinylated peptide biotin-EHSSKLQKC served as a molecular recognition element. It was self-assembled on the surface of the wells of a 96-well microtiter plate modified with gold nanoparticles (AuNPs). In parallel, streptavidin-coated magnetic beads (strep-MBs) were modified with the enzyme invertase and then added to the peptide-modified wells upon which the modified strep-MBs bind to the peptide via biotin/streptavidin interaction. If a sample containing PSA is placed in the well, PSA will cause the cleavage of the peptide, and a respective quantity of invertase-modified strep-MBs will be released. The supernatant containing the invertase-modified strep-MBs is taken out and sucrose is added which is enzymatically cleaved by invertase. The concentration of the glucose formed after 1 h is quantified with a personal glucose meter. It is linearly related to the concentration of PSA in the range from 80 pg to 7 nga (TM) mL(-1). The detection limit is 30 pga (TM) mL(-1) and the relative standard deviation is 3.7 % (at a level of 500 pga (TM) mL(-1) and for n = 7). The method was successfully applied to the determination of PSA in spiked real human urine. Due to its simplicity, sensitivity and selectivity, this bioassay offers a promising approach to the detection of PSA and other biomolecules.
• 卷号：182
• 期号：9-10
• 是否译文：否