Optically Programmable Plasmon Enhanced Fluorescence-Catalytic Hairpin Assembly Signal Amplification Strategy for Spatiotemporally Precise Imaging

Key Words:SILICA

Abstract:Signal amplification strategies with spatiotemporallyhigh sensitivity can provide more accurate information and hold greatpromise for improving the accuracy of disease diagnosis. Herein, a808 nm near-infrared (NIR) light-activated plasmon enhancedfluorescence-catalytic hairpin assembly (PEF-CHA) signal amplifica-tion strategy was proposed for spatiotemporally controllable preciseimaging of miRNA in vitro and in vivo with ultrasensitivity. Theproposed 808 nm NIR light-activated PEF-CHA signal amplificationstrategy is constructed through combining up-conversion photo-control and PEF technologies with CHA. It is worth noting that thelaser irradiation-induced overheating effect could be effectivelyalleviated by using Nd3+-sensitized upconversion nanoparticles(UCNPs) to convert 808 nm NIR light to ultraviolet (UV) light,which is almost nondestructive to cells or tissues. In addition, nonspecific activation as well as false positive signals can be effectivelyavoided. Moreover, the detection limit can be reduced by approximate 38 times thanks to the high sensitivity of the proposedstrategy. Furthermore, we demonstrate that the 808 nm NIR light-activated PEF-CHA signal amplification strategy can be expandedto sensitive and activatable imaging of intratumoral miRNAs in living mice, showing feasible prospects for precise biological andmedical analysis

Volume:94

Issue:13

Translation or Not:no