论文成果
A glassy carbon electrode modified with a composite consisting of reduced graphene oxide, zinc oxide and silver nanoparticles in a chitosan matrix for studying the direct electron transfer of glucose oxidase and for enzymatic sensing of glucose
- 发布时间：2021-03-15
- 点击次数：
- 关键字：Biosensor; Bioassay; Enzymatic assay; Nanocomposite Microwave synthesis; In-situ reduction; Hydrazine reduction; Direct electrochemistry; Amperometry
- 摘要：The authors describe the fabrication of a nanocomposite consisting of reduced graphene oxide, zinc oxide and silver nanoparticles by microwave-assisted synthesis. The composite was further reduced in-situ with hydrazine hydrate and then placed, along with the enzyme glucose oxidase, on a glassy carbon electrode. The synergistic effect of the materials employed in the nanocomposite result in excellent electrocatalytic activity. The Michaelis-Menten constant of the adsorbed GOx is 0.25 mM, implying a remarkable affinity of the GOx for glucose. The amperometric response of the modified GCE is linearly proportional to the concentration of glucose in 0.1 to 12.0 mM concentration range, and the detection limit is 10.6 A mu M. The biosensor is highly selective, well reproducible and stable.
- 卷号：183
- 期号：5
- 是否译文：否