负载型非晶合金催化剂Ni-Co-P/γ-Al_2O_3的制备及其结构和催化性能表征
关键字：OVARIAN-CANCER; SURFACE; HYDROGEL
摘要：It is crucial to construct antifouling electrochemical biosensors with long-term stability and high sensitivity for the detection of biomarkers in complex biological environments to prevent the unspecific adsorption of proteins and other biomolecules. Herein, a glassy carbon electrode electropolymerization with polyaniline (PANI/GCE) was further modified with a copolymerization (PSN) of zwitterionic sulfobetaine methacrylate (SBMA) and hydrophobic N-isopropyl acrylamide (NIPAM) by simple hydrophobic interaction to construct an antifouling biosensor. PSN forms a stable self-assembled monolayer on the PANI/GCE surface and exhibits good antifouling performance in serum due to the advantages of hydrophobic anchoring and the antifouling performance of zwitterionic polymers. Ascribe to the large specific surface area and excellent conductivity, the present biosensor can detect CA125 in undiluted samples in the range of 0.01-1000 U mL-1 with a limit of detection of 2.7 mU mL-1 (3 sigma/k). Both with good biocompatibility and biological stability, PSN modified sensing surface realizes a long-term antifouling for 15 days in a buffer solution. The present work provides a simple strategy for the direct analysis of CA125 in human serum without a complicated construction of biosensors, indicating the potential application in clinical diagnosis.
卷号：387
期号：
是否译文：否
