An optical DNA logic gate based on strand displacement and magnetic separation, with response to multiple microRNAs in cancer cell lysates

Release time:2023-10-19 Hits:

Key Words:Logic circuit; Magnetic separation; DNAzyme; Spectrum; Colorimetric

Abstract:A battery of logic gates, "YES", "AND" and "OR", are constructed using magnetic beads (MBs) modified by DNA which consists of a substrate strand (S) and a signal strand on which the logic operates. Inputs stemming from micro-RNA (which represent three cancer biomarkers) take the place of signal DNA. The released signal strand selfassembles into the hemin-G-quadruplex complex (DNAzyme) that catalyzes a blue-green dye (ABTS(+)) from the precursor ABTS. This dye (quantified at a wavelength of 414 nm) represents the output signal for the various logic gates. The method allows quantitative detection ofmicroRNA of three kinds of logic gates in the range of 5 nM-500 nM with detection limits of 3.8 nM, 4.9 nM, 5.4 nM. Boolean logic circuitry is also achieved following the principles of multilevel strand displacement. Based on strand displacement and magnetic separation, this work demonstrates the possibility of designing a logic system using micro-RNA in live cell lysate as inputs, and its potential application in DNA computation and cancer diagnosis.

Volume:184

Issue:8

Translation or Not:no