青岛科技大学  English 
聂广明
赞  

教师拼音名称:N G M

手机版

访问量:

最后更新时间:..

Fabrication of a simple and sensitive QDs-based electrochemiluminescence immunosensor using a nanostructured composite material for the detection of tumor markers alpha-fetoprotein

关键字:

摘要:A simple and sensitive label-free QDs-based electrochemiluminescence (ECL) immunosensor for detecting tumor markers alpha-fetoprotein is reported. This probe is fabricated using a nanostructured composite material (PICA-MWNT) obtained by the direct electrodeposition of indole-6-carboxylic acid (ICA) monomers and carboxylic groups terminated multiwalled carbon nanotubes (MWNTs) in one step. The obtained composite material, with 2-aminoethanethiol modified CdSe nanoclusters as luminescent particles, has a larger surface area and a considerable amount of functionalized carboxylic acid groups. Thus, QDs/PICA-MWNT will probably display good biocompatibility, high ECL intensity, and stability, which is promising for the enhancement of detection signals and synergistic improvement of sensitivity. The ECL signals are logarithmically linear with the concentration of alpha-fetoprotein in a wide determination range from 0.002 to 2000 ng mL(-1), and the corresponding detection limit was 0.4 pg mL(-1). This proposed ECL sensor exhibits high stability, good selectivity, and reproducibility, which offers a new insight into the fabrication of immunoassays for detecting other relevant biomarkers, and it has the potential for a reliable point-of-care diagnostics of tumor or other diseases.

卷号:2

期号:47

是否译文:

崂山校区 - 山东省青岛市松岭路99号   
四方校区 - 山东省青岛市郑州路53号   
中德国际合作区(中德校区) - 山东省青岛市西海岸新区团结路3698号
高密校区 - 山东省高密市杏坛西街1号   
济南校区 - 山东省济南市文化东路80号©2015 青岛科技大学    
管理员邮箱:master@qust.edu.cn