A novel electrochemiluminescence biosensor based on Ru (bpy)32 -functionalized MOF composites and cycle amplification technology of DNAzyme walker for ultrasensitive detection of kanamycin

Key Words:METAL-ORGANIC FRAMEWORKS; ELECTROGENERATED CHEMILUMINESCENCE; LUMINOL

Abstract:An electrochemiluminescence (ECL) sensing platform for ultrasensitive and highly selective detection of kanamycin (KANA) was developed based on the prepared Ru(bpy)32 -functionalized MOF (Ru@MOF) composites by hydrothermal synthesis and Ag -dependent DNAzyme. In this sensor, the stem-loop DNA (HP) with the ferrocene (Fc) was used as substrate chain to quench the ECL emi generated by the Ru@MOF. Using the specific recognition effect between KANA and the KANA aptamer (Apt) and the DNAzyme dependence on Ag , the KANA aptamer as the pendant strand of the DNAzyme was assembled on Ru@MOF/GCE with the aptamer. When both Ag and KANA were present simultaneously, KANA specifically was binded to KANA aptamer as a pendant chain. Subsequently, Ag -dependent DNAzyme walker continuously cleaved the HP chain and released the modified end of Fc to restore the ECL signal of Ru@MOF composites, thus achieving selective and ultrasensitive detection of KANA. The constructed KANA biosensor exhibits a wide detection range (30 pM to 300 mu M) accompanied by a low detection limit (13.7 pM). The KANA in seawater and milk samples are determined to evalute the practical application results of the sensor. This ECL detection strategy could be used for detecting other similar analytes and has broad potential application in biological analysis.

Volume:659

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Translation or Not:no