- Self-powered organic semiconductor photoelectrochemical strategy via CBT-Cys reaction for HSV-1 DNA detection
- 点击次数：
- 关键字：CHARGE-TRANSFER
- 摘要：Perylene-based organic semiconductors with large it-conjugated system are promising materials in field of photoelectrochemical assay. Herein, PTC-NH2Br2, as a perylene-based organic semiconductor polymer nano- material, was synthesized from perylene-3,4,9,10-tetracarboxylic dianhydride (PTCDA), ethylenediamine (EDA) and 1,7-dibromo-3,4,9,10-perylenetetracarboxylic dianhydride (PTCDA-Br2). It possessed typical characteristics of stable photocurrent response from-0.2 V to 0.3 V, and excellent PEC performance compared with PTC-NH2 and PTCDA-EDA-DAP, which are the other derived polymers of PTCDA. A click reaction actuated organic semiconductor self-powered photoelectrochemical assay was fabricated, which proceeded at 0 V. The presence of target triggered CBT-Cys reaction, which in turn activated target recycling rolling circle amplification (RCA) and produced plenty of single-stranded DNA to combine with Cas12a-crRNA complex, and activated trans-cleavage activity of Cas12a. During this process, the target recycled. The trans-cleavage activity of the activated CRISPR/ Cas12a system led to the degradation of ssDNA and the release of alkaline phosphatase (ALP). Through target recycling and CRISPR/Cas12a system, the detection limit of this photoelectrochemical assay exhibited 2.0 aM, and it was further applied to the biological analysis of actual samples with satisfactory results.
- 卷号：209
- 期号：
- 是否译文：否
