王鲲鹏

副教授

副教授 硕士生导师

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学历:博士研究生

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A fluorescent probe based on cyclochalcone for detecting peroxynitrite

发布时间:2024-12-24 点击次数:

关键字:CHEMILUMINESCENT PROBE; HYDROGEN-PEROXIDE; IN-VITRO; CELLS
摘要:A novel cyclic chalcone fluorescent probe C-PN was synthesized to detect ONOO-. After reaction with peroxynitrite, the double bond of C-PN in the cyclic chalcone structure was disconnected, which caused the change of intramolecular charge transfer (ICT) effect, emitting blue fluorescence and quenching orange red fluorescence. Visible to the naked eye, the color of the probe solution changed. The probe showed low sensitivity (detection limit = 20.2 nm), short response time (less than 60 s) at low concentration of ONOO-, good visibility, and good selectivity and stability for ONOO-.
卷号:23
期号:6
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