Release time:2025-07-14  Hits:

• Key Words:LIVING CELLS; ENDOGENOUS PEROXYNITRITE; HYDROGEN-PEROXIDE; NITRIC-OXIDE; METABOLISM; DYE
• Abstract:Peroxynitrite (ONOO-) plays a pivotal role in ious physiological and pathological processes. The sensitive and specific detection of ONOO- is of paramount significance for early diagnosis, treatment, and prognosis of diseases. In this study, a near-infrared (NIR) fluorescent probe was developed for the detection of ONOO- with rapid responsiveness (less than 100 s). As the concentration of ONOO- increases, the ratio of fluorescence intensities exhibited a robust linear relationship with ONOO- concentration. Additionally, the probe demonstrated the ability to selectively detect changes in ONOO- levels, with a limit of detection (LOD) value of 13 nM. Importantly, the probe was successfully employed to detect endogenous ONOO- in living cells and mouse models. In conclusion, the fluorescent probe exhibits significant potential for elucidating disease processes induced by ONOO- .
• Volume:239
• Issue:
• Translation or Not:no