Release time:2023-10-19  Hits:

• Key Words:HCLO; ACID; PEROXYNITRITE; DESIGN
• Abstract:The level of HClO/ClO- in mitochondria is essential to keep the normal function of mitochondria. Therefore, it is meaningful to accurately and quickly monitor ClO- in mitochondria. In this work, a new triphenylamine-based fluorescence probe PDTPA was designed and synthesized, in which pyridinium salt and dicyano-vinyl group were introduced as mitochondria targeting site and reaction site for ClO-. The probe showed high sensitivity and fast fluorescence response (<10 s) in the detection of ClO-. Moreover, the probe PDTPA had good linearity in a wide concentration range of ClO- and its detection limit was calculated as 10.5 & mu;M. Confocal fluorescence images demonstrated that the probe could target mitochondria and track the fluctuations of endogenous/ exogenous ClO- levels in the mitochondria of living cells.
• Volume:299
• Issue:
• Translation or Not:no