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Abstract:The purification method of peroxidase was studied. A novel and simple purification process was used. R<sub>z</sub> value of soybean peroxidase reached 1.32 and the recovery of enzyme activity was 65% after purification. In this new process, the enzyme solution was purified by the ammonium sulphate-acetone cooperation precipitation, the acetone fractional precipitation and zinc sulfate precipitation. The ammonium sulphate-acetone cooperation precipitation, namely, the system of organic solvent-inorganic salt two phases, was adopted in the process. This is a simple and effective purification method of biomacromolecule.
Volume:33
Issue:6
Translation or Not:no