Dual-Mode Scattering Nanoprobes for Imaging Hydrogen Sulfide in Living Cells

发布时间：2023-10-19 点击次数：

• 关键字：FLUORESCENT-PROBE; RAMAN TAGS; NANOPARTICLES; PROLIFERATION; TARGET; H2S
• 摘要：Hydrogen sulfide (H2S), a gaseous signaling agent, was considered to play an essential role in the regulation of many biological systems. Herein, we design a surface-enhanced Raman scattering (SERS) and plasmon resonance Rayleigh scattering (PRRS) dual-mode nanoprobe for real-time imaging of H2S in living cells. To realize the dual-mode imaging, we fabricated a core-shell nanostructure with built-in electromagnetic hot spots by embedded Raman dyes between a gold nanostar (AuNS) core and a silver shell. When exposed to sulfide, the Ag shell will rapidly transform into Ag2S, resulting in the variation of PRRS caused by the change of the dielectric constant and the reduction of the SERS signal resulted from the blocking effect of Ag2S. There was well-defined linearity between the dual signals and the sulfide concentrations from 1 to 100 nM. The limit of detection (LOD) was 87 pM for the SERS mode and 53 pM for the PRRS mode, respectively. Complementary performances were proclaimed between the two proposed sensing modes, i.e., the PRRS mode sensing based on single nanoprobes can provide local concentration information of H2S in single cells, while the SERS signal can directly provide the average concentration information of H2S in single cells. This nanoprobe has been successfully utilized in real-time monitoring of H2S levels in living cells.
• 卷号：4
• 期号：7
• 是否译文：否