Highly selective ratiometric electrogenerated chemiluminescence assay of DNA methyltransferase activity via polyaniline and anti-fouling peptide modified electrode

发布时间：2021-03-15 点击次数：

• 关键字：Gold;Fluorescence - Ligands - Nanoclusters - Zinc - Zinc compounds
• 摘要：Herein, a zinc ion (Zn²⁺)-triggered aggregation induced emission enhancement (AIEE) fluorescence "on-off-on" nanoswitch was fabricated for inorganic pyrophosphate (PPi) and inorganic pyrophosphatase (PPase) activity detection. Dual ligand functionalized Au NCs were utilized as the substrate of the AIEE nanoswitch. The introduction of Zn²⁺ can cause Au NCs aggregated along with the enhanced fluorescence. After the addition of PPi, aggregated Au NCs disaggregated along with decreased fluorescence due to the competitive combination between PPi and Zn²⁺ (on-off). When PPase was introduced, PPi was hydrolyzed and release Zn²⁺, resulting in aggregated Au NCs along with enhanced fluorescence again (off-on). On the basis of this, highly selective and sensitive detection PPi (liner range from 0.1 to 300 μM) and PPase activity (liner range from 0.1 to 10 mU) can be achieved. The detection limits are 0.04 μM for PPi and 0.03 mU for PPase, respectively. Furthermore, the as-prepared Zn²⁺-triggered AIEE nanoswitch was successfully used for quantitative analysis of PPase activity in human serum with satisfactory spiked recoveries, and applied for the inhibitors screening.<br/> © 2019 Elsevier B.V.
• 卷号：1079
• 期号：*
• 是否译文：否