Platinum-Selenopeptide Interfaces in Support of High Fidelity Electrochemical Biomarker Quantification in Complex Biological Matrices

Key Words:GOLD; MDM2; GLUTATHIONE; THIOL; INHIBITORS; BIOSENSORS; STABILITY; THERAPY; CARBON; SENSOR

Abstract:The real world applications of conventional antifouling biosensors based on gold-thiol (Au-S) interfaces are hampered by the progressive competitive displacement of key functionality by ubiquitous biothiols. To overcome this limitation, we introduce here novel platinum-selenium (Pt-Se) interfaces. Thiol displacement tests, antifouling analyses, and density functional theory calculations confirm markedly improved interfacial stability. This was then leveraged through the application of a seleno-multifunctional peptide platform, tailored to the detection of murine double minute 2, in biological samples. A derived amperometric sensing platform exhibited a notably lower detection limit and more accurate target quantification than that supported by analogous Au-S and Pt-S interfaces. We believe that this work broadens the scope of electrochemical sensor construction and holds significant promise for the development of high-fidelity impactful bioassay platforms.

Volume:96

Issue:38

Translation or Not:no