Ultralow fouling electrochemical detection of uric acid directly in serum based on phase-transited bovine serum albumin and conducting polymer

Key Words:PROTEIN

Abstract:Electrochemistry with antifouling sensing interfaces that effectively resist the adsorption of nonspecific biomolecules provides a powerful mean for the accurate and sensitive detection of disease biomarkers in complex biofluids. However, there are few strategies to acquire a stable and solid antifouling coat-ing on any substrate by a simple way. Herein, a simple one-step assembly method has been adopted to construct phase-transited bovine serum albumin (PTB) antifouling layers. Prior to construction of the an-tifouling layers, the poly(3,4-ethylenedioxythiophene) (PEDOT) doped with 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (ionic liquid, IL) were firstly electrodeposited on bare electrodes, en-dowing good conductivity and catalytic capability for the developed sensor. Subsequently, with the assist of tris(2-carboxyethyl)phosphine (TCEP), the disulfide bonds of bovine serum albumin (BSA) were re-duced to form PTB, which can be coated on the PEDOT-IL modified electrode to construct an antifouling electrochemical senor (PTB/PEDOT-IL/GCE) for the detection of uric acid (UA) in human serum. The UA sensor demonstrated a good linear range from 1.11 mu mol/L to 798.9 mu mol/L, with a high sensitivity of 0.556 mu A mu mol L-1 cm-2. The combination of conducting polymers with one-step assembly of PTB offers a universal and reliable method for the modification of ious electrodes to determine target molecules in complex human body fluids.(c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.

Volume:34

Issue:12

Translation or Not:no