A label-free electrochemical immnunosensor based on signal magnification of oxygen reduction reaction catalyzed by uniform PtCo nanodendrites for highly sensitive detection of carbohydrate antigen 15-3

Key Words:EFFICIENT CATALYST; IMMUNOSENSOR; PERFORMANCE; GRAPHENE; ELECTROCATALYSTS; NANOCOMPOSITES; NANOCATALYSTS; IMMUNOASSAY; NANOFLOWERS; LUMINOL

Abstract:Developing a highly sensitive immunoassay for tumor biomarkers is particularly important in bioanalysis and early disease diagnosis. In this work, a simple one-pot solvothermal method was developed for controllable synthesis of well-dispersed PtCo alloyed nanodendrites (PtCo NDs) by using L-carnosine as the co-structure-directing agent. The PtCo NDs had a large specific surface area and provided abundant active sites available for electrocatalytic oxygen reduction reaction (ORR). Based on the highly enhanced currents of the ORR, a novel label-free electrochemical immunosensor was fabricated for highly sensitive assay of carbohydrate antigen 15-3 (CA15-3). The sensor showed a wide linear range of 0.1-200 U mL(-1) and a low limit of detection (LOD) down to 0.0114 U mL(-1) (S/N = 3), in turn exploring its application to diluted human serum samples with satisfactory results. This study provides a feasible platform for monitoring other tumor markers in clinical diagnosis. (C) 2021 Elsevier B.V. All rights reserved.

Volume:1176

Issue:

Translation or Not:no