Ultrasensitive dual-signal ratiometric electrochemical aptasensor for neuron-specific enolase based on Au nanoparticles@Pd nanoclusters-poly(bismarck brown Y) and dendritic AuPt nanoassemblies

Key Words:Palladium alloys;Binary alloys - Diagnosis - Gold - Gold nanoparticles - Graphene - Iron compounds - Nanoclusters - Nanoparticles - Neurons - Organometallics - Printing machinery - Probes - Reduced Graphene Oxide - Synthesis (chemical) - Tumors

Abstract:Neuron-specific enolase (NSE) is viewed as a tumor marker of small cell lung cancer (SCLC) with high diagnostic sensitivity and specificity, whose facile and sensitive detection is critical for clinical diagnosis. In this work, core-shell Au nanoparticles @Pd nanoclusters-poly(bismarck brown Y) (Au@Pd-P(BBY)) and hierarchically dendritic AuPt nanoassemblies (AuPt NAs) were synthesized independently by a one-pot aqueous method. A dual-signal ratiometric electrochemical aptasensor was developed for ultrasensitive determination of NSE based on ferrocene grafted Au@Pd-P(BBY) (Fc-g-Au@Pd-P(BBY)) and reduced graphene oxide/thionine decorated AuPt NAs (rGO/Thi/AuPt NAs). Under optimal conditions, the changes in the peak currents linearly correlate to the NSE concentrations in the wide range of 0.0001―50.0 ng mL^–1 with ultralow detection limit (0.03 pg mL^–1, S/N = 3). The aptasensor was explored in a diluted human serum sample with acceptable results, showing its great potential in clinical diagnosis and treatment.<br/> © 2020 Elsevier B.V.

Volume:311

Issue:

Translation or Not:no