Simple one-pot aqueous synthesis of 3D superstructured PtCoCuPd alloyed tripods with hierarchical branches for ultrasensitive immunoassay of cardiac troponin I

Key Words:Copper alloys;Antibodies - Catalyst activity - Chemical detection - Cobalt alloys - Diagnosis - Immunosensors - Palladium alloys - Platinum alloys - Tumors

Abstract:By integrating the amplified electrochemical signals and effective capture of antibodies together, advanced multimetallic superstructured nanocrystals endow label-free immunosensors promising applications in early diagnosis and monitoring of diseases. Herein, four-metallic PtCoCuPd hierarchical branch-like tripods (HBTPs) were directly synthesized by a green one-pot aqueous method without any seed or organic solvent involved, which were applied to construct a novel label-free immunosensor for detecting cardiac troponin I (cTnI). The specific hierarchical micro/nanostructures greatly improved the immobilization of antibodies and enhanced the catalytic activity for K<inf>3</inf>Fe(CN)<inf>6</inf>, which would effectively amplify the electrochemical signals, thereby improving the detection sensitivity. Under the optimal conditions, the as-developed immunosensor exhibited a wide linear range (0.001–100.0 ng mL⁻¹) and a low detection limit (0.2 pg mL⁻¹, S/N = 3) for the assay of cTnI. The immunosensor provides a powerful platform for quantitative detection of cTnI, which can be explored to detect other tumor markers in actual sample analysis.<br/> © 2019 Elsevier B.V.

Volume:145

Issue:

Translation or Not:no