Three dimensional sea-urchin-like PdAuCu nanocrystals/ferrocene-grafted-polylysine as an efficient probe to amplify the electrochemical signals for ultrasensitive immunoassay of carcinoembryonic antigen

Key Words:Antigens;Biocompatibility - Chemical detection - Diagnosis - Gold nanoparticles - Immunology - Immunosensors - Iron compounds - Mammals - Nanocrystals - Organometallics - Phase locked loops - Polyethylene oxides - Shellfish - Ternary alloys

Abstract:A novel sandwich-like immunosensor was efficiently fabricated for detection of carcinoembryonic antigen (CEA) with three dimensional sea-urchin-like PdAuCu nanocrystals (PdAuCu NCs)/ferrocene-grafted-polylysine (Fc-g-PLL) as the label of secondary antibodies (Ab<inf>2</inf>) and Au nanoparticles (Au NPs) as the substrate material. Herein, PdAuCu NCs were directly synthesized with polyethylene oxide (PEO) as a growth-directing agent by a facile one-step aqueous method without any organic solvent. Meanwhile, Fc-g-PLL was obtained by covalent linkage of Fc with PLL via Schiff-base reaction. The well-dispersed PdAuCu NCs by Fc-g-PLL have the enlarged surface area, enhanced catalytic properties and superior biocompatibility to amplify the current signals. The resultant immunosensor shows linear relationship of the electrochemical responses with the CEA concentrations within a broader linear range (0.001–100.0 ng mL^–1) and a lower detection limit (0.23 pg mL^–1, S/N = 3). Furthermore, the immunosensor was explored for practical assay of CEA in human serum samples with accredited results. The novel immunoassay provides a feasible platform for early medical diagnosis.<br/> © 2019 Elsevier B.V.

Volume:132

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Translation or Not:no