Ratiometric fluorescent probe with signals from two near-infrared channels for esterase activity and its application in biological imaging

Release time:2024-01-19 Hits:

Key Words:ESIPT; CELLS; AIE

Abstract:Esterase serves as a crucial biomarker for the diagnosis of tumors due to its over in cancer cells. Ratiometric fluorescent probe has the advantage of having two distinct emi wavelengths, which allows for the elimination of the effects of light intensity and optical path length by using the fluorescence intensity ratio between the two emi wavelengths. In this work, a novel near-infrared (NIR) fluorescent probe P1 for ratiometric monitor esterase activity was developed. Compared with ordinary ratiometric probes, both emi wavelengths of this probe are in the NIR region, which can effectively eliminate ious fluctuations and blur-riness in image contrast and achieve precise quantitative analysis. P1 itself shows a fluorescence emi at 717 nm. After incubated with esterase, a new emi peak at 822 nm emerged due to esterase's catalysis, and the fluorescence intensity at 717 nm steadily decreased, which achieved the highly sensitive detection of esterase with a detection limit calculated as 0.25 mU/mL. What's more, P1 showed high selectivity for esterase and was successfully applied for biological imaging in living cells and in vivo.

Volume:395

Issue:

Translation or Not:no