A water-soluble fluorescent probe for the determination of ?-glutamyltransferase activity and its application in tumor imaging

Release time:2023-10-19 Hits:

Key Words:GAMMA-GLUTAMYL-TRANSPEPTIDASE; LIVING CELLS; PHOSPHATASE-ACTIVITY; TRANSFERASE; DIAGNOSIS; ASSAY

Abstract:gamma-glutamyltransferase (GGT), an important tumor marker, is highly expressed in tumor tissues, and precise detection of its activity provides a vital indicator for the diagnosis and treatment. In this work, a "lighting-on" probe (TCF-GGT) was elaborated to detect endogenous GGT with high selectivity and sensitivity. Dicyanome-thyldifuranyl (TCF-OH) was employed as the fluorescence reporter and short peptide glutathione (GSH) worked as the GGT-active trigger, the introduction of which prevented the initial proton transfer of TCF-OH contributing to a blank sensing background. A bright red fluorescence could be switched on upon GGT catalytic hydrolysis, avoiding the potential interference from background. There displayed an excellent water-solubility, and little organic solvent was required during the exploration, which otherwise avoided the potential damage to enzyme and organism. TCF-GGT has been proved to be workable at cellular and organism level with highly effective imaging and a short metabolic cycle, which is expected to offer an alternative solution or reference to the early diagnosis and treatment of tumor.

Volume:253

Issue:

Translation or Not:no