An electrochemical biosensor for alpha-fetoprotein based on carbon paste electrode constructed of room temperature ionic liquid and gold nanoparticles

Release time:2021-03-15 Hits:

Key Words:Electrochemical immunoassay; alpha-Fetoprotein (AFP); Carbon paste electrode (CPE); Gold nanoparticle; Room temperature ionic liquid (RTIL); O-Aminophenol (OAP)

Abstract:A novel and effective electrochemical immunosensor for the rapid determination of alpha-fetoprotein (AFP) based on carbon paste electrode (CPE) consisting of room temperature ionic liquid (RTIL) N-butylpyridinium hexafluorophosphate (BPPF(6)) and graphite. The Surface of the CPE was modified with gold nanoparticles for the immobilization of the alpha-fetoptotein antibody (anti-AFP). By sandwiching the antigen between anti-AFP on the CPE modified with gold nanoparticles and the secondary antibody, polyclonal anti-human-AFP labeled with horseradish peroxidase (HRP-labeled anti-AFP), the immunoassay was established. The concentration of AFP was determined based on differential pulse voltammetry (DPV) signal, which was generated in the reaction between O-aminophenol (OAP) and H(2)O(2) catalyzed by HRP labeled oil the sandwich immunosensor AFP concentration Could be measured in a linear range of 0.50-80.00 ng mL(-1) with a detection limit of 0.25 ng mL(-1). The immunosensor exhibited high sensitivity and good stability. and would be valuable for clinical assay of AFP. (c) 2009 Elsevier B.V. All rights reserved.

Volume:78

Issue:3

Translation or Not:no