青岛科技大学  English 
聂广明
赞  

教师拼音名称:N G M

手机版

访问量:

最后更新时间:..

Label-free DNA detection based on a novel nanostructured conducting poly(indole-6-carboxylic acid) films

关键字:Label-free DNA detection; Conducting polymer; Hybridization; Indole-6-carboxylic acid; Nanostructure

摘要:A simple and label-free electrochemical sensor for detection of DNA hybridization event was obtained based on a novel nanostructured conducting polymer, poly(indole-6-carboxylic acid). This precursor polymer with high electroactivity can be easily prepared by direct electrochemical polymerization of indole-6-carboxylic acid in acetonitrile containing tetrabutylammonium tetrafluoroborate. An 18-mer amino-substituted oligonucleotide (ODN) probe was covalently grafted onto the polymer in a one-step procedure. The performance of this label-free electrochemical DNA sensor was investigated by using cyclic voltammetry (CV) as a readout method. This simple sensor can discriminate one-base mismatched and non-complementary ODN sequences and can be regenerated after a dehybridization step. The dynamic determination range for complementary target ODN was from 3.5 x 10(-10) mol L-1 to 2.0 x 10(-8) mol L-1 and the corresponding detection limit was 5.79 pmol L-1. (c) 2009 Elsevier B.V. All rights reserved.

卷号:139

期号:2

是否译文:

崂山校区 - 山东省青岛市松岭路99号   
四方校区 - 山东省青岛市郑州路53号   
中德国际合作区(中德校区) - 山东省青岛市西海岸新区团结路3698号
高密校区 - 山东省高密市杏坛西街1号   
济南校区 - 山东省济南市文化东路80号©2015 青岛科技大学    
管理员邮箱:master@qust.edu.cn